Application
This Anti-Cytokeratin Epithelial Antibody, clone AE1 is validated for use in IH(P) for the detection of Cytokeratin Epithelial.
Research CategoryCell Structure
Research Sub CategoryCytokeratins
Immunohistochemistry on unfixed frozen sections and formalin-fixed paraffin embedded tissues. Antigen retrieval is recommended. HEIR or protease treatment can be used.
Western Blotting: AE1 recognizes LMW cytokeratins from the acidic subfamily including: Clone AE1 recognises the 56.5, 50, 50′, 48 and 40kD human cytokeratins of the acidic subfamily.
Optimal working dilutions must be determined by end user.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Human epidermal keratin
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Linkage
Replaces: 04-588
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Liquid in 0.05M Borate Buffered Saline, 0.15M NaCl, 0.09% Sodium Azide, pH8.2.
Specificity
Recognizes most of the acidic (Type I) keratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. MAB1612 produces heterogeneous staining within a given epithelium; the detailed staining pattern varies depending on the differentiated and/or growth state of individual cells.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
This product has met the following criteria to qualify for the following awards: